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The numbers of emerged seedlings from the soil surface were counted daily up to 14 days when seedling emergence was thought to be complete.
Seeds were sown at 3 cm, 5 cm, 7 cm, and 10 cm soil depth and the number of emerged seedling form soil surface were counted daily up to 14 days after sowing.
Cells remaining on the upper surface of the filter membrane (non-migrant) were gently scraped off using a cotton swab and the cells on the lower surface were counted randomly in 3 scopes at least.
Fig. 4 Seedling emergence percentage at 6 days after sowing (a), mesocotyl (b) and coleoptile length (c) of 54 CSSLs (SL); 12 seeds of 54 CSSLs, Nipponbare and Kasalath were sown at 5 cm soil depth and incubated and the numbers of emerged seedling from the soil surface were counted.
Only single flask-shaped caveolae clearly associated to the cell surface were counted.
The cells that had migrated through the pores to the lower filter surface were counted in three random high power fields in each well (magnification 100×).
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The number distribution of nanoparticles collected on the filter surface was counted visually and converted to the distribution in the air using existing filtration models for Nuclepore filters.
Liver tissue was observed with the naked eye, and the number of visible tumors in the liver surface was counted.
In this study, the number of metastasis nodules on the lung surface was counted.
Mice were then sacrificed, and the number of black macro-metastases on the lung surface was counted.
After 48 hours, the number of metastatic foci on the lung surface was counted with a dissecting microscope.
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