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Vertical position of the ePaddle module with respect to the ground surface was fixed during the experiments for eliminating influence of wheel's sinkage on measured soil reaction forces.
In order to obtain a homogeneous DBD discharge, the stainless steel tube with a previously polished external surface was fixed at the center of the glass tube using PTFE spacers.
The lower surface was fixed in methanol and stained with May-Grunwald stain (Sigma) according to manufacturer's protocol.
The lower membrane surface was fixed by 4% formaldehyde, stained with Hoechst 33 342 and counted under a fluorescence microscope.
After another 24-hour culture, the number of cells that migrated to the lower surface was fixed by crystal violet and examined.
The fractured biological surface was fixed with carbon on the stub and immediately introduced into the SEM (Phenom G2 Pro, PhenomWorld) in a frozen chamber (−20°C) for 30 min.
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The proposed sliding surface is fixed time convergent.
Cells that had migrated to the bottom surface were fixed in 4% paraformaldehyde and counterstained with DAPI (Vector Laboratories, USA).
An image is drawn with tusche (a carbon pigment in liquid form) and litho crayon before the printing surface is fixed, moistened, and inked in preparation for printing.
Non-migrating and non-invading cells were removed from the upper surface of the membrane, whereas cells that adhered to the lower surface were fixed with paraformaldehyde 4% for 15 min.
The simulations are based upon the assumptions of the well known van der Drift model in which the growth rate of each surface is fixed only by its crystallographic orientation.
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