Sentence examples for surface sterilisation from inspiring English sources
Needles were assessed individually for lesion formation (present/absent) and plated on P10ARP medium after surface sterilisation (30 seconds in 70% ethanol and two washes in sterile deionised water).
For sizes 1 3 plastic disposable containers (150 ml volume) with screw cap lids (Sterilin, UK) were used to culture the capsules following surface sterilisation; for size 4 and 5 Dilu vials (27 ml volume, Alpha Laboratories, UK) with flip tops were used.
Our method uses a combination of salt flotation, immunomagnetic separation (IMS), and surface sterilisation of oocysts prior to DNA extraction, with subsequent use of the transposome-based Nextera XT kit to generate libraries for sequencing on Illumina platforms.
In the main phase salt-floated oocysts were treated similarly, but all available oocysts were used (ranging from 3.9×10 to 1.9×10, see Table 2) and dissociated oocysts adjusted to 200 μL with RO water prior to surface sterilisation.
Oocysts were dissociated from the magnetic beads into 1.5 ml microfuge tubes (Elkay, Basingstoke, UK), and the volume adjusted to 1 ml with RO water prior to surface sterilisation.
After surface sterilisation, seedlings were cultivated using a modified Hoagland nutrient solution either (1) on hydroponics according to Smeets et al. [ 76], but using purified sand or (2) on rockwool plugs.
Because surface-sterilisation was not applicable in our study design, we could not distinguish between viruses on the surface of the eggs (transovum transmission) or within the eggs (transovarian transmission).
We conclude that surface modification by sterilisation could prove to be a useful tool at the final stage of scaffold production to enhance cell contact, phenotype or function.
New developments are devoted to surface cleaning and low temperature sterilisation.
Most cementless femoral stems are produced from wrought titanium alloys [ 3], by using conventional manufacturing processes that include rolling, forging, machining, surface modification, finishing, cleaning, and sterilisation.
They were ca. four cm long; we rid the plant rhizomes of all roots and immersed them for 15 min. into a 20% solution of Savo (sodium hypochlorite) for sterilisation of their surface.
