Sentence examples for surface protein analysis from inspiring English sources

A number of molecules functioning in cellular movement (381), proliferation (567), development (552), death and survival (520), and cell-to-cell signaling (290) were detected having top biological functions in LESCs and several of these were confirmed by flow cytometric surface protein analysis.

Cell surface protein marker analysis demonstrated that Huv-iPS cells expressed the pluripotent markers Tra-1-60, Trand81 and SSEA-4, and had lost expression of the endothelial marker CD31.

For cell surface protein expression analysis, cells were harvested and washed with phosphate buffered saline, and incubated with antibodies for 1 h at room temperature.

Surface protein level analysis found higher positivity for CD146/MCAM, CD47 and CD117/c-kit in LESCs compared to CECs, showing a pattern typical for stem cells and higher multipotency in the earlier cell type (Additional file 1: Figure S1).

Flow cytometric surface protein level analysis found a significantly higher number of positive cells for ICAM1 in CECs (56.19 ± 12.46%) than in LESCs (4.37 ± 7.63%) (p = 0.0001) (Additional file 1: Figure S1).

Surface protein level analysis found same expression levels of CD29/Integrinβ1 and CD44/HCAM in the two cell types, while CD54/ICAM1 positivity was higher in the CECs (Additional file 1: Figure S1).

The results of the western blot analysis and the relative levels of MerTK protein expression were also confirmed by flow cytometric analysis of surface protein expression (data not shown).

According to our analysis, the surface protein of the HIV virion, gp120, has a consistently low predicted disorder value across various strains of lentiviruses (data not shown).

As shown by Northern blot analysis and surface protein expression analysed by flow cytometry, IFN-γ-treatment decreased PECAM-1-gene-expression in isolated SECs (sinusoidal endothelial cells) and mononuclear phagocytes (MNPs) in parallel with an increase in ICAM-1-gene-expression in a dose and time dependent manner.

Transmembrane proteins and secreted proteins were annotated as cell surface proteins for further analysis.

The same batch of cells was used for extracting cell surface proteins and for analysis of conditioned media and whole cell lysate proteins.