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The smears persist across the surface of the spot and contribute to additional signal intensity in the Cy3 channel.
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Exciting radiation with power of 1 2 mW was focused on the sample surface to the spot of 1 μm in diameter.
A very simple and effective method for the confirmation of substrate depletion is by scraping the surface of the hydrolysis spot with something pointed such as the tip of a mechanical pencil.
The signal intensity that we measure on the array is assumed to be proportional to the ratio of bound-to-total surface of the peptide spot, S i.
This can only be explained by contamination with an un-cleaved protein located on the surface of the excised spot or contamination introduced into the sample prior to enzymatic digestion.
Trim or clip the hair from the surface of the hot spot.
However, the surface of the black spotted bib interacted with diquat-treatment (F1,58.4 = 8.12, P = 0.006; Fig. 4).
Looking up towards the shining surface of the sea, I spot groups of shy unicorn fish, pointing their humped foreheads like bowsprits.
You can exploit the clear water glitch where you can see everything very clearly right between the surface of the water to spot the fin gleam easier.
Among them, D 1 represents the image data vector of spot 1. D 2 represents the image data vector of spot 2. α is the curved surface of light spot 1. β is the curved surface of the light spot 2. Of course, in general, the solution scheme can be extended to any light spot intensity analysis after changing the light diversity direction of the two points.
Dr. Juergens said in a telephone interview that Dr. Tschinkel was "looking for the wrong termites and you could easily overlook the ones that were actually living" deep beneath the surface of the red sandy spots, feasting on grass roots to keep the patches of land free of vegetation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com