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This technique, developed in the 1930s, enabled researchers to examine synapses and other neuronal structures in unprecedented detail, but can only capture images at the surface of the specimen being studied.
The action of the electron beam stimulates emission of high-energy backscattered electrons and low-energy secondary electrons from the surface of the specimen.
Initial surface of the specimen before ion etching corresponds to zero depth on the depth profile.
The laser beam from the light source scans the surface of the specimen.
The measurement of carbonation depth was conducted by breaking up the splitting surface of the specimen.
It employs finely focused electron beam to image the surface of the specimen.
An edge surface of the specimen was coated with white spray to highlight the crack tip.
Division surface of the specimen mainly presented calcification and partially red soft tissues (Fig. 2c).
Some of these nanoparticles aggregate and then get deposited on the surface of the specimen [8].
When thermal cracking occurred, the cracks always nucleated at the surface of the specimen.
The approach generates accurate predictions of the strain distribution on the surface of the specimen.
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