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The spherulites located closer to the surface of the membrane have fewer limitations to grow toward and on the surface of the membrane.
Cells on the lower surface of the membrane were considered as invaded cells.
Un-migrated cells from the upper surface of the membrane were removed with a cotton swab.
Afterward, cells on the lower surface of the membrane were fixed with 4% paraformaldehyde and stained with crystal violet.
Using filipin, we observe the rapid movement of the fluorescence from the surface of the membrane to the cytoplasm after treatment with C4.
The efficiency of membrane phosphorylation (the conversion of PI to PI4P) was determined from the biosensor's fluorescent signal on the surface of the membrane.
After culturing for 24 hours, cells on the lower surface of the membrane were stained with 0.1% crystal violet and photographed using the Carl Zeiss Axio Observer microscope.
After culturing for 36 hours, cells on the lower surface of the membrane were stained with 0.1% crystal violet and photographed using the Carl Zeiss Axio Observer microscope.
Invaded cells (cells on the lower surface of the membrane) in five microscope areas (200 × magnification) were counted and imaged by a microscope (Leica, Germany).
How does aspartate binding to the sensing domain at the outside surface of the membrane translate into a change in kinase activity at the membrane cytosol interface?
In contrast, peptides with ≥3 lysines reside along the surface of the membrane.
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