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For the sitting drop method, the protein drop sits directly on a surface of the crystallisation plate above the crystallisation condition.
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The glaze melting and crystallisation ranges and the kinetic parameters of the crystallisation process were determined by differential thermal analysis (DTA).
While previous studies have examined the effects of protein construct, protein purity, or crystallisation condition ingredients on protein crystallisation, few have examined the effect of the crystallisation plate.
The crystallisation condition (85 µl) was placed in the reservoirs of the crystallisation plates using a Biomek® 2000 Laboratory Automation workstation (Beckman Coulter, California, USA).
Many studies have examined the effect of the crystallisation condition ingredients or the plate geometry on the rate of equilibration of the protein drop with the crystallisation condition and both can markedly affect the quality of the crystals [4], [5].
Each drop was built by adding 200 nL of the crystallisation condition to 200 nL of the protein solution.
The thermal scans of all the compositions exhibited two crystallisation (Tc1 and Tc2) and melting peaks (Tm1 and Tm2); in addition, the intensity of the crystallisation peaks decreased and became less defined with increasing boron content.
This is because new mesoporous (radius >2 nm) surfaces are created by the crystallisation of calcite minerals or by an increase in mesopores due to carbonation shrinkage (at the expense of micropores (radius <2 nm).
Due to the influence of two silicon sources and the small surface area of the geopolymer bulk, in the hydrothermal crystallisation process, the amorphous three-dimensional network structures of the Na2O Al2O3 4SiO2 geopolymers were converted into large-sized ANA single crystals.
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