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For the invasion assay, the upper chamber surface of the basement membrane of the Transwell was coated with Matrigel (Corning).
These cells make contact with the outer surface of the basement membrane by slender cytoplasmic extensions called pedicels (foot processes).
This model (Fig. 12) shows that the surface of the basement rock is inclined to the northeast direction, and the thickness of the sedimentary rocks varies consequently from about 90 m southward to about 105 m at the central part of the area under the point of intersection between the two models over the graben G3.
The results of 2D magnetic modelling indicated that, the depth to the surface of the basement rocks varied from 45 m to about 110 m, and proved that the variation in the magnetic field in the area was caused only by the striking faults rather than the variation in the lithological constituents of the basement.
The second mechanism involves the negatively charged sialo-protein-rich electrical charge on the surface of endothelial cells covering the inner surface of the basement membrane.
The epithelial cells formed a single layer and loosely covered the surface of the basement membrane.
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The thickness of the epithelial layer was determined on days 7 and 60 by 48 measurements per biopsy at intervals of 0.05−1 mm of the vertical distance from the epithelial surface to the basement membrane by means of "Leica Q Win" software (Leica Microsystems, Wetzlar, Germany).
In vivo endothelial cells (ECs) are located under the microenvironment of both surface topography of the basement membrane and the mechanical loading resulting from blood flow.
Thick clastics began to accumulate upon the erosion surface of the acoustic basement at ca. 5 Ma (e.g., Kamata 1989; Sasada 1987).
The present seismic study revealed that the older asymmetric half-graben on the basal drag fault is thickest along the southern margin of the bay. Figure 3 delineates an irregular surface of the acoustic basement of the hanging wall (annotated as a 'wedge tip deformation' in the figure).
The podocyte wraps around the capillary wall on the outer surface of the glomerular basement membrane with its extended inter-digitating foot processes.
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