These discs were placed on the surface of the agar plates already inoculated with selected bacterial organisms.
After 6 days, plantlets growing on the surface of the agar were transferred to fresh growth media.
After the incubation, all plates were observed for bacteria colony growth on the surface of the agar medium (Additional files 2, 3, 4, and 5).
The B. subtilis penetrons formed on the surface of the agar hydrogel were incubated at 30°C for 72 hrs, and were collected from the surface of the agar hydrogel with 2 ml of 200 mM NaCl and a cell scraper.
The B. subtilis penetrons formed on the surface of the agar hydrogel were incubated at 30°C for 72 hrs, and were then collected from the surface of the agar hydrogel with 2 ml of 200 mM NaCl and a cell scraper.
The plates were inoculated by dipping a sterile swab into the inoculum suspension adjusted to the turbidity of a 0.5 McFarland standard (106 cells/mL) and streaking it across the surface of the agar in four directions.
Sterile double distilled water was added to the plates to collect conidia from the surface of the agar, and the suspension was filtered through sterile Miracloth (Millipore, Billerica MA, USA) and counted in a haemocytometer.
Subsequently, the PHB-producing and PHB-non-producing cells were scraped from the surface of the agar plates and suspended in 100 µL of phosphate-buffered saline (PBS, pH 7.2, 20 mM).
The edge of a sterilized coverslip was dipped into a cell suspension of the bacterial strain and then gently pressed onto the surface of the agar, creating a thin inoculation line.
The disk diffusion was performed by placing different types of disks including bark extract (50 μL), synthesized silver nanoparticles (50 μL/10, 25, 50 mg/L), standard antibiotic (erythromycin 50 μL) and synthesized silver nanoparticles with standard antibiotic on the surface of the agar in plates and incubated for 24 h at 37 °C.
The mycelium is made of bent-over hyphae, without any aerial hyphae (hyphae that extend above the surface of the agar).
