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Furthermore, the membrane-bound Klotho could not be observed at the surface of kidney cells in mammals, but only as a diffuse expression in the cytoplasm.
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The optic fibre was placed directly on the surface of the kidney and ultrasound gel was gently placed between the ultrasound probe and the kidney.
Using the LI-COR imaging system, immunostaining for RALDH1 was located in the medullary portion of the control mouse kidney (Figure 3A) with lesser amounts present in the renal cortex near the surface of the kidney.
Briefly, the left kidney was exteriorized; a small transverse incision was made through the capsule on the ventral surface of the kidney, near the inferior pole.
It arises on both sides from the concave surface of the kidney known as the hilum.
The bisected surface of the kidney consists of the lighter-colored outer cortex and the darker-appearing inner medulla.
Everything collects sort of at what's at the bottom of this diagram but it would be on the inside or medial surface of the kidney and it's in its anatomic position.
The antidiuretic action of vasopressin is thought to depend upon its binding to the outer surface of the kidney tubule, resulting in an increase in the uptake of sodium from the urine into the tubule cells and, concurrently, an increase in the uptake of water.
Finally, a laser Doppler probe (0.25 mm fiber separation, 780 nm wavelength; Perimed AB, Järfälla, Sweden) was sutured to the surface of the kidney for cortical measurements, and a needle laser Doppler probe (0.15 mm fiber separation, 780 nm wavelength) was inserted 10 12 mm into the kidney for medullary measurements.
This image is a composite of three Hematoxylin & Eosin stained images from the surface of the kidney to the outer portion of the renal medulla.
Superficial lymph nodes are greatly enlarged and oedematous, and foci of lymphocytic infiltration may be seen on the cut surface of the kidney.
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