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The ramp is inclined with an angle of 9° compatible with the observed average slope topography (note that the angles ∼15° 17° measured in the alcove would rather correspond to the scar inclination rather than to the surface of flow).
Diluted filaments were attached to the surface of flow cells and visualized in TIRF as described above for the nucleation assay.
Complexes were allowed to dissociate for approximately three minutes, after which a solution of wtDR1/HAY308A complexes (∼2.5 µM) pre-incubated with SEH or SEA at a 1∶1 molar stoichiometry was injected over the surface of flow cell 1 or 2 respectively for another ten minutes.
Center: fluorescence confocal images of the coverslip surface of flow cells containing rhodamine-labeled microtubules (red) and 60 nM GFP-fused test proteins (green).
After activation with streptavidin (SA), flow cells 1 and 3 were retained as blank references, and DNA was coated on the SA surface of flow cells 2 and 4. Surface testing, regeneration buffer scouting, and the mass transport limitation test were performed before the kinetics experiments, as described previously.
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It was inspiring to see teens, usually more at home on the tough surface of city streets than the uncertain surface of flowing water, up at dawn and venturing into such unknown territory.
As a typical metagenome, the biofilm growing on the surface of flowing AMD belongs to a low-complexity and relatively self-contained ecosystem [ 19].
On the surface of the flow cell, hundreds of millions of fragments of DNA had gathered into hundreds of millions of microscopic colored spots.
To quantify the binding we first immobilized sonicated fibrils on the surface of one flow cell of a CM5 biosensor chip and added monomeric α-Syn protein (Supplementary Fig. S9a).
By adding ratchet gratings to the surface of micromixers flow patterns could be manipulated.
Fluorescently labeled microtubules were fixed to the glass surface of a flow chamber by the use of polyclonal tubulin antibodies (ab1289, Acris Antibodies GmbH, Germany) as described in [22] or by use of the biotin-streptavidin system (see above).
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