In agreement with the results in Figure 2, in ndc80Δ 490-510 and ndc80-7A mutant cells, CEN3 was captured by the lateral surface of a spindle-pole MT at 35°C with similar kinetics as wild-type cells; by contrast, subsequent sister CEN3 separation on the spindle proceeded more slowly compared with wild-type cells, indicative of a delay in sister CEN3 biorientation.
Figure 1C (inset) shows a well-formed liquid tip in contact with a surface, with a spindle tip diameter of ≈200 μm.
Its functioning is based essentially on sliding of conical surfaces located in a spindle and in a series of holding pins radially disposed around it.
Its functioning is based essentially on sliding of conical surfaces, located in a spindle and in holding pins.
The viscous turbine drives the spindle by exerting viscous traction forces directly on the smooth surface of the spindle.
Liu, et al. [97], installed two FBG temperature sensors (position 1 and 3) and four thermal resistors (position 1, 2, 3, and 4) on the bearing support surface of the spindle (shown in Figure 19).
Although the spindle thus appears to have a variable distribution over the cortical surface across time, it is possible that the total set of cortical areas active at some point in generation of a spindle burst is consistent across spindle bursts.
Each tool of a spindle head has its own spindle speed.
However, the probability for the spindle to be in a given range of angles is proportional to a «stripe» of the surface of a sphere (Fig. 5A top), not to an arc at the periphery of a circle (Fig. 5A bottom) since the spindle can take any orientation in a 3D space and is not limited to a 2D plane.
Highly sulfated HS was located in the border zone between denser areas and more normal looking alveolar parenchyma in basement membranes of blood vessels and airways, that were immuno-positive for perlecan, as well as on the cell surface of spindle-shaped cells in the alveolar interstitium.
After the initial 31 days of primary culture, UCB-MSCs adhered to a plastic surface and presented a small population of single cells with a spindle shape.
