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In this work, blood cells were labelled with magnetic nanoparticles and fluorescent probes and aligned along ferromagnetic lines deposited by lithographic techniques on an optically transparent surface of a chamber under a magnetic field (Fig. 14).
The pin repeatedly delivered ∼12.5 nl/spots of sample solution onto the glass surface of a chamber slide in a pre-arranged format (Figure 1A far-left panel), yielding a series of spots with the size ∼0.5 mm×0.5 mm (Figure 1A far-right panel).
The ability of the β2M-expressing transfectants to migrate across the surface of a chamber was also analysed 24 h after seeding.
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Chemical polishing is applied to clean the extruded surface of a copper chamber.
Fluorescently labeled microtubules were fixed to the glass surface of a flow chamber by the use of polyclonal tubulin antibodies (ab1289, Acris Antibodies GmbH, Germany) as described in [22] or by use of the biotin-streptavidin system (see above).
Biotin-labeled microtubules were fixed on the surface of a flow chamber that was first incubated with 2 mg/ml BSA-biotin (Sigma-Aldrich Co., St . Louis MO, USA) subsequently with 1 mg/ml Streptividin in BRB80+ buffer and 20 µM paclitaxel.
To calibrate the bead intensity as a function of z position, the surface of a sample chamber was decorated with 0.5-µm diameter beads.
This PEG-treated quartz slide was placed as the bottom surface of a microfluidic chamber to be used as the imaging surface of our prism-type total internal reflection fluorescence (TIRF) microscopy (based on IX-71, Olympus).
Myosin activity was tested by a classical motility assay where myosins bound to a nitrocellulose coated glass surface of a perfusion chamber (tebu-bio [Cytoskeleton Inc.]) propel actin filaments.
Here we give an overview of a technology developed in our laboratory, which relies upon simple micro- or nanofabricated structures in combination with "bio-friendly" lipid bilayers, to align thousands of long DNA molecules into defined patterns on the surface of a microfluidic sample chamber.
Briefly, the microscope design permitted selective fluorescence excitation of molecules immobilized on the surface of a glass flow chamber (Smith et al., 2013) using three lasers at wavelengths 488 nm, 532 nm, and 633 nm.
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