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The surface localisation of the SceA-GFP fusion protein on vegetative cells and spores was confirmed by Western blot, immunofluorescence microscopy and flow cytometry.
Binding of the FERM domain to receptors has also been proposed to assist in cell surface localisation of several cytokine receptors [4], [5], [6], [7], [8], [9].
Changes in the expression and surface localisation of integrins during EMT have been documented [ 14, 15].
For example, Homer, Norbin (neurochondrin) and Tamalin (GRASP1) act as chaperones to increase cell surface localisation of mGluR5.
The phosphorylation status of the mature 170 kD P-glycoprotein and the surface localisation of this glycoprotein showed the strongest correlation with functionality.
This vector targets the scFv to the endoplasmic reticulum where the scFv antibody is retained, thus preventing the cell surface localisation of CA125/MUC16.
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The EGF-induced cell-surface localisation of AnxA2 is obvious phenomenon as EGF stimulates the pp60c-Src kinase activity via EGFR (Jorissen et al, 2003).
Receptor-mediated cell-surface localisation of the various components of the PAS (for example, uPA, plg, and plg activator inhibitor type 1 [PAI-1] and type 2 [PAI-2]) is critical for the spatial and temporal regulation of proteolysis.
AnxA2 is a major substrate of pp60c-Src kinase and increases the phosphorylation of AnxA2 at Tyr-23, a site known for increasing cell-surface localisation of AnxA2 (Gerke and Moss, 2002).
First, we compared the cell-surface localisation of AnxA2 in triple-negative and Herceptin-resistant breast cancer cell lines with non-tumourigenic epithelial cells and ER+, PR+, and HER2+ breast cancer cell lines.
Clustering of the collagen binding integrins has been implicated in the cell surface localisation, and thus activity of MT1.
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