Sentence examples for surface library from inspiring English sources
Design of the pZ surface library followed Nord and co-workers.
Electrophysiological examination of these candidates by two-electrode voltage clamp in Xenopus oocytes identified a clone from the pZ surface library, N5 (for Library N, clone 5), in which all 13 surface residues were changed in the parent protein pZ, that had potent effects on both yeast rescue and channel function.
With virtually no overlap (e.g., only 5% of ribotypes were shared between seawater and dead coral surface libraries) between coral-associated and seawater bacteria [14], and vertical transmission of bacteria seemingly absent in at least one coral species [26], one important question arises: How (and when) do corals acquire the complex microbiota commonly found in adult colonies?
In the work described in this paper we constructed 13 yeast surface libraries in which the C-terminal part of the EF-loop of the CH3 domain of IgG1-Fc was randomized and additional residues were inserted.
We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens.
Accordingly, we used a yeast surface display library of an entire naïve repertoire of human scFv antibodies to select for binding to a NAC peptide.
A yeast surface display library of Fn3 was created in which three solvent-exposed loops were diversified in terms of amino acid composition and loop length.
The cell surface displayed library was screened against V-domain Ig suppressor of T-cell activation (VISTA), an important immune-checkpoint regulator.
The discovery and characterization components have previously been characterized but in order to fully utilize the capabilities of affinity reagents from our yeast surface display library, efforts were focused on developing a production component to obtain purified, soluble, and active scFvs.
Because the idea behind the surface display library that generated N5 was to exploit a folded scaffold and yield a protein that retained folded structure, we first asked whether the extensive mutational changes perturbed the structure of the pZ scaffold.
Recently discovered and characterized fluorogen activating proteins (FAPs) are based on scFvs, selected for activation of otherwise nonfluorescent dyes from a yeast surface display library and subsequently affinity matured for tighter binding.
