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14, 15 FreeSurfer-generated output was reviewed for accuracy by the consensus of 2 raters, who excluded 25 of 803 cases (3.1%) because of inaccurate surface labels.
By contrast, experiments with other dyes produced a clear inhibitory effect, particularly cell surface labels such as PKH26 and 67 (data not shown), presumably because they reduce RBC recognition and invasion.
Cell dyes were chosen to have minimal emission overlap with the best performing DNA dyes and to label erythrocytes cytoplasmically rather than on the erythrocyte surface as has been used previously (3), reasoning that surface labels may reduce invasion efficiencies and therefore reduce the sensitivity of the assay.
Similar(57)
Surface labelling was performed with FITC-labelled antibodies to CD14 (DAKO, Denmark, 1 100 dilution) for 45 min directly on adherent viable PBMLs under ice bath.
The surface labeled H+ Σ0) is called the future Cauchy horizon of Σ0.
We propose that aptamer surface labeling of the nanoparticles could enhance the selectively delivery of p-gp siRNA into the metastatic breast cancer cells.
Animal cap cells were surface labeled with cleavable biotin, induced with activin, and plated on FN.
Remarkably, dysbindin depletion visibly inhibited turnover of surface labeled D2 receptors (Fig 2C, right panel).
Surface labelling and co-immunoprecipitation was performed as previously described for γδ T cells [64].
Cell surface labeling and affinity purification is a standard method to selectively extract cell surface proteins [18].
CD44, the cell surface receptor for hyaluronate [13], had been utilized as a surface label to select CSC.
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