Sentence examples for surface labeling from inspiring English sources

For the cell surface labeling experiments, 2×106 splenocytes or liver leukocytes were incubated with the corresponding Abs and tetramer for 30 min at 4°C in staining buffer (PBS with 2% FBS and 0.1% NaN3).

Total co-culture is used for cell surface labeling.

We verified appropriate ciliary localization of engineered receptors by anti-Flag Alexa555 surface labeling.

As well, cell surface labeling efficiencies of DIFO, DIBO, and BARAC directly correlated with their relative reactivities.

If a certain protein is present on the cell surface, labeling with biotin results in a change in electrophoretic mobility.

Early studies used metabolic labeling and surface labeling techniques with SDS-PAGE to detect altered protein patterns [ 1- 3].

Furthermore, membrane-bound CtsB was detected on PyMT tumor cells by using a cell surface labeling approach[ 6d] with biotinylated inhibitor NS-196.

Figure S6 (available on the Blood website; see the Supplemental Materials link at the top of the online article) shows FACS analysis of lymphocyte cell-surface labeling.

In all fluorescent activated cell sorter (FACS) experiments, cells were pre-coated with anti mouse CD16/CD32 (BD Pharmingen,) to block unspecific binding, and T cells were identified by cell-surface labeling with APC-labeled anti-Thy1.2 (BD Pharmingen).

Within 3 minutes of treatment with LY294002, the cell-surface labeling of GFP-Grp1-PH was ablated, and its distribution throughout the cell became predominantly cytosolic (Figure 6B).

We observed cell-surface labeling of sialic acids at 8.5 hpf, during gastrulation.