Sentence examples for surface kb from inspiring English sources
Co-staining with the Golgi marker Giantin showed very little colocalization of surface Kb or Kb-OVA complexes with Golgi in DCs from KbWT and Δ7 mice.
As little as 10 nM bruceantin inhibited peptide supply as judged by expression of surface Kb MHC I molecules (data not shown).
The viral infection slightly increased the surface Kb expression in both CMT.64 and CMT.1,2/Kb cells (Fig. 3 C right panel).
Notably, overlapping of EEA-1 and Kb-OVA complexes without surface Kb (indicated by pink color) was significantly higher (p = 0.002) in Δ7-derived DCs (Figure 5D).
DCs from Δ7 mice showed an intermediate phenotype, with ∼20% of surface Kb molecules being internalized at 30 minutes of chase time (Figure 2A and B).
Surface Kb molecules that lack exon 7, by contrast, appear to traffic readily into early endosomal compartments, but are significantly delayed in their ability to traffic into late endosomes or lysosomes.
In order to assess how entry of surface-derived Kb molecules into endocytic compartments coincides with exogenous antigen loading, Kb surface-labeled DCs were pulsed with soluble OVA protein for 6 hours to allow simultaneous Kb-FITC internalization and OVA uptake and antigen processing.
By contrast, early endosomes from ΔY-derived DCs contained no detectable surface-derived Kb or Kb-OVA complexes (Figure 5A).
Similarly, KbWT-derived DCs demonstrated extensive triple overlap of surface-derived Kb and OVA-loaded Kb molecules with LAMP-1-positive compartments (Figure 5A).
Prior to assessing the entry of surface-derived Kb molecules into endocytic compartments, a competition assay using flow cytometry was designed.
However, co-localization analysis of Kb-OVA complexes with the ER marker Grp78 showed that only a minor fraction of Grp78-positive compartments contained such complexes, and these compartments also colocalized with surface-derived Kb molecules (unpublished data).
