Sentence examples for surface inoculation from inspiring English sources
Also, GFP-labeled E. coli O157 H7 strains were detected on the surface of lettuce leaves at least 26 days after leaf surface inoculation in an environtron [24].
A range of surface inoculation levels were evaluated, but higher inoculum (e.g. ≥ 103 cfu/site) of C. difficile spores produced growth that was too numerous to count when sampled using Rodac plates.
For the final volume of liquid for both leaf and soil surface spray inoculation was 1 mL (water + inoculant) per pot containing two plants, and inoculants were diluted with sterile distilled water at 1 1000 (v:v) and 1 7500 (v:v) for spraying maize and wheat, respectively.
For leaf and soil surface spray inoculation, an aerograph atomizer was employed to mimic the action of a spraying equipment.
For soil surface spray inoculation, plant shoots were covered with plastic bags to make sure that the inoculant reached only the soil surface.
Recent studies in determination of infection and colonization of lettuce roots by a GFP-expressing lettuce isolate of V. dahliae showed that germ tube emergence from the infected root surface following inoculation extended longitudinally along root epidermal cells, and distinct appressoria formed within cell junctions and directly penetrated adjoining cells [10], [12].
The malt extract agar was sterilised with 20 µL of 200 mg/L Ampicillin to kill any possible bacteria before 2 drops of inoculum was put on the malt extract agar in a petri dish, spread to cover the surface with sterile inoculation loop under aseptic condition and incubated at 24 °C for 5 days.
Using this CryoSEM technique, the inoculation surface of the frozen leaf segments was viewed directly while being maintained at – 135°C.
Conidia germinated between 12 and 36 hpi and hyphae grew in all directions across the leaf surface from the inoculation site.
Additionally, a disruption and dilution sampling method, as described below as a previously optimized MSSA quantification standard protocol, was used as supplementary quantification methods to confirm the ability for the RODAC plates to recover cells from the surface after spray inoculation.
Using logistic regression, results from this method were used to model the binary responses of Listeria on surfaces with realistic inoculation levels.
