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It is conceivable that the digital media might provide an extensive and/or mobile surface for a display of the exhaustive representation, but even so the limits of human vision might restrict its perception as one single complete visual percept as one whole.
It was assumed that only 1% of the drop contents is retained on the chip surface for membrane display.
The inflated cortical surfaces used for displaying results in Figure 2 were created using Caret (Van Essen C et al. 2001; Van Essen DC et al. 2001).
Isothermal adsorption process has been known as a robust method to generate biomimetic surfaces suitable for displaying and investigating the effects of ECM functional domains on neural differentiation [ 11].
As aggregates comprise a large number of individual protein molecules, there is the potential for the surface to display a "repetitive" array of epitopes that may be seen by the immune system as resembling the external surfaces of pathogens, triggering the pattern recognition receptors (Toll-like receptors) on APCs.
A previously developed expression system for surface display of heterologous proteins on the surface of Staphylococcus carnosus employs the secretion signals from a Staphylococcus hyicus lipase and the cell wall anchoring part of Staphylococcus aureus protein A (SpA) to achieve surface display of expressed recombinant proteins.
In recent years, the LysM domains have been widely applied for various biotechnological purposes such as in the preparation of oral influenza vaccine and for surface display of heterologous proteins on bacterial cell surfaces (Bosma et al. 2006; Okano et al. 2008; Shao et al. 2009; Hu et al. 2010; Saluja et al. 2010; Xu et al. 2011).
Saccharomyces cerevisiae strain EBY100 (MATa AGA1 GAL1-AGA1 URA3 AGA1 GAL1-AGA1 URA3his3Δ200 pep4::HIS3 prb1Δ1.6R can1 GAL) was ura3 52r surface display, and strain YVhis3Δ200α pep4::GALDH-PDI1::LEU2 ura3–52 trp1 leu2Δ1 his3Δ200 pep4::HIS3 prb1Δ1.6R can1 GAL) was used for protein surfaceon.
Constructs for surface display of NCXCL-Aga2C fusion proteins were generated using DNA assembly methods and enzymes described above.
Directing heterologous antigen for surface display or secretion may increase humoral responses.
Autotransporters have been successfully applied for surface display of various heterologous proteins.
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