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Specifically, the levels of Fc receptor mediated-phagocytosis and the levels of FcγRs surface expression were evaluated by flow cytometry analysis, while quantitative real-time PCR analysis was utilized to examine the mRNA expression levels of FcγRs.
All cells with down-modulated HLA-ABC surface expression were also eGFP+.
All the NTCP variants tested were examined for NTCP expression, and comparable levels of cell surface expression were confirmed.
To determine if the in vivo HB22.7 half-life and the xenograft's CD22 cell surface expression were related, a FACS-based assay to measure HB22.7 levels was used.
Notably, the loss of stimulation of basal activity and reduced surface expression were not correlated with changes in total channel expression levels.
The kinetics of UL18 surface expression were shown to be regulated by two motifs in the cytoplasmic tail of UL18, causing ER retention and internalization.
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However, other cell lines (e.g., CAKI-2) expressed Jak2 protein but still no surface expression was observed.
Surface expression was performed on COS-7 cells transiently expressing HA-tagged Kir6.2 and SUR1 (Zerangue et al, 1999).
Its surface expression is indispensable for antigen presentation [29].
Surface expression is dependent on total channel expression, surface-trafficking efficiencies and/or stability.
Receptor surface expression was determined with specific monoclonal antibodies by FACS analysis.
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