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However, other cell lines (e.g., CAKI-2) expressed Jak2 protein but still no surface expression was observed.
Surface expression was performed on COS-7 cells transiently expressing HA-tagged Kir6.2 and SUR1 (Zerangue et al, 1999).
Integrin αIIbβ3 surface expression was elevated on the platelets from CD82 null mice and they displayed enhanced adhesion and tyrosine kinase signaling on fibrinogen.
Furthermore Als5pV326N cell surface expression was consistent with proper folding and processing.
Receptor surface expression was determined with specific monoclonal antibodies by FACS analysis.
Phosphotidylserine cell surface expression was assessed using Annexin-V FITC kit (Becton Dickinson, UK).
Furthermore, CD35 and CD55 RBC surface expression was not associated with age.
Severely impaired cell surface expression was also observed for MT1-G166E MT1-I212T212T mutants.
LAIR-1 surface expression was also undetectable on platelets stimulated by collagen or ADP (data not shown).
Most efficient reduction of receptor cell surface expression was found when Cys341 alone or both Cys residues were mutated [37].
Early addition of IL-15Rα neutralizing mAbs to PB-HP resulted, within four days, in inhibition of CD56 while CD34 surface expression was preserved (Fig. 5, D4).
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