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However, biomembrane surface cultivation also had an obvious limitation.
In this work, both biomembrane surface cultivation and shake cultivation were used to produce the pigment.
Biomembrane surface cultivation was conducted at 28 °C in a thermostat shaker.
Fig. 1 Shake cultivation and biomembrane surface cultivation for production of red pigments of P. novae-zelandiae.
The procedure for biomembrane surface cultivation was the same as that of shake cultivation except that the rotary speed of the thermostat shaker was at 50 rpm.
Amino acids at a dosage of 0.5 g/L were added to the modified Czapek Dox liquid medium, and the fermentation process was the same as that of biomembrane surface cultivation described previously.
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In some production systems phosphorus (P) and nitrogen (N) accumulate at or near the soil surface and cultivation is one option for addressing the legacies of previous nutrient applications.
The impact of silver and copper nanoparticles on the growth rate of P. herpotrichoides mycelium was determined by surface fungal cultivation on an agar medium with a solution called biogenic metal nanoparticles.
Tilman [1] observed that doubling of world agricultural production over the 35-year period, from 1961 to 1995, was accompanied by an increase of only 10% of the surface under cultivation.
They are built to provide a larger surface area for cultivation on hillslopes and to aid farming production in the long term.
The selected sub-watersheds were chosen according to the percentage of surface area under cultivation and classified as low (0 19%), moderate (20 59%) or high (>60%).
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