Supra-threshold methods are used in instances where the stimulus is easy to perceive, and are used to discriminate between stimuli, such as discrimination between sounds [14].
In addition, intracellular cysteine may also react with TQ Green to afford an overestimated GSH concentration using the imaging method (vide supra).
By plotting the λexmax for the successive dilutions as a function of the polymer concentration, CMC values of 0.2, 0.1, and 0.4 mg·mL–1 were obtained for L 5000, H 2000, and H 5000, respectively, which are in agreement with the CMC values determined by the surface tension method (vide supra).
From these two EC50 values we were able to construct an isobologram and theoretically determine combinations of low concentrations of both drugs that would potentially have a supra-additive effect (synergism) (see Methods).
RNA was isolated using the same method described above (via supra).
First, because it allows avoiding obvious cortical lesions and stimulating the cortical surface at supra-threshold intensity (see 'Materials and methods' section and Casali et al., 2010) and second (and most important) because it ensures high test-retest reproducibility when TMS-evoked potentials are performed longitudinally (Lioumis et al., 2009; Casarotto et al., 2010).
(On the question of hypotheses and the relation of this passage to the hypothetical method of the Phaedo, see §15 supra).
All genotyping methods were performed at the Emerging Bacterial Pathogens Unit, WHO/IUATLD Supra-National Reference TB Laboratory, San Raffaele Scientific Institute (FCSR).
Relative gene expression was analyzed by the 2-ΔΔCT method (User Bulletin #2: ABI Prism 7700 Sequence Detection System vide supra (http://www3.appliedbiosystems.com/).
IRE is a member of a family of non-thermal methods to ablate tissue with electrical pulses, which includes electrochemotherapy (ECT) [7], [15] [18] and supra-poration [19] [21].
