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This exemplifies the diversity of the MSC as a functionally supportive cell in multiple environments.
Thirteen percent (13%) were derived from the stem cell supportive cell line AFT024, derived from the murine fetal liver microenvironment (http://stromalcell.princeton.edu).edu
The endosteal HSC niche contains osteoblasts as the main supportive cell type for maintenance of hematopoiesis [ 6, 7].
Therefore, the formation of 3D constructs with cells capable to differentiate into functional cardiomyocytes and supportive cell phenotypes in the right proportions is the goal in tissue engineering and cardiac regenerative medicine.
Highly purified EPCR++CD150+CD48−CD45+ (E-SLAM) HSCs have previously been found to be highly efficient (>90%) at making colonies under supportive cell culture conditions when a strict viability gate is set [12,13].
To support the concept of pluripotentiality of VSELs in vitro, we have also successfully developed several co-culture condi-tions where VSELs, if plated over supportive cell lines, may expand/differentiate into a variety of somatic cell types [ 17].
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Second, MSCs and HSCs may act as each other's supportive cells, and reciprocally promote tissue-forming and vascular support functions.
Malignant glioma is a primary brain tumor that originates from the supportive cells of the brain called glial cells.
Traditionally, glial cells have been considered merely as supportive cells keeping the neurons together, but this view is changing [13].
RSC96 Schwann cells are the main supportive cells of the peripheral nervous system and are responsible for the myelination of axons.
Moreover, tumor stem cells and tumor supportive cells, such as cancer associated fibroblasts and cancer associated macrophages, are left unaffected by this approach.
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