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The Far-UV CD experiments have also shown that there is no significant change in the secondary structure of AdoMetDC and the mutants upon putrescine binding, supporting the crystal structures.
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This notion is supported by the crystal structures of the Fc glycoforms bearing sequentially truncated glycans ((G2F 2, (G0F 2, (M3N2F 2 and (MN2F 2, G: galactose; M: mannose; N: GlcNAc; F: fucose) in which the (G2F 2 glycoform shows the longest Pro329-Pro329 Cα distance of 33.7 Å whereas the (MN2F 2 glycoform the shortest distance of 21.9 Å (Krapp et al., 2003).
This model is strongly supported by the crystal structures of the archaeal, yeast and human exosome [ 23- 26].
The OB-fold prediction is now supported by the crystal structures of the HIN domains of IFI16 and AIM2 [ 56, 57].
To obtain further support for the crystal structures described above, we performed biochemical characterization of HI-6 and K027 using mAChE and its mutants.
This conclusion is supported by the crystal structure of another mutant that has two hydrophobic residues substituted for the two non-hydrophobic residues.
Topological analysis suggest that Int188 is composed of sixteen beta-sheets together with four alpha-helices (Figure 1A), which can be supported by the crystal structure (Figure 1B).
This is supported by the crystal structure of the nucleoprotein from VSV that shows the neighboring lobe interactions provide more stability than the positively charged residues of the RNA binding cavity [22].
Hence, it seems most likely that the correct binding mode of the synaptotagmin-1 C2B domain is that determined in solution by NMR spectroscopy [17], which is now further supported by the crystal structure of the synaptotagmin-7 C2B domain presented here (see discussion).
Our results are supported by the crystal structure of BtpA.
This proposal is further supported by the crystal structure of H50S, which contains two alternating conformations of A2062 [ 4].
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