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Transcripts with retained introns may be annotated in cases where there are both supporting cDNA and RNA-Seq coverage data.
In total, 93 apple predicted genes with supporting cDNA sequences were identified between 0.8 Mb to 2.0 Mb on LG 16 (Additional File 5; figure S4).
The remaining Arabidopsis genes were classified as expressed genes or hypothetical genes according to the gene set downloaded from TAIR7 release [ 31] which had at least one supporting cDNA and/or EST.
Although there is no supporting cDNA or EST evidence for our predicted gene structure, the small intron sizes (e.g. LCCL is separated by 135 bp from the downstream CTLD) and well-conserved CTLDs, suggest that the prediction may be correct if the corresponding region was correctly assembled.
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Supporting cDNAs were identified for at least 32 unique members in P. taeda (Additional file 1), a number close to that described in P. abies (Additional file 1) and Arabidopsis [ 63- 65], higher than the number described in P. pinaster and P. glauca (Additional file 1) [ 65, 66], and lower than the number described in Oryza sativa, Populus trichocarpa and Brassica rapa [ 63, 65, 67].
This supports cDNA-AFLP data indicating the induction of genes with similar functions during withering [ 25].
In addition, transient expression of Pokemon in MCF-7 cells induced survivin upregulation in a parallel manner to Pokemon levels), supporting the cDNA microarray data from stable clones.
bp: Base pair; BS: Bootstrap support; cDNA: Complementary DNA; gDNA: Genomic DNA; HGT: Horizontal gene transfer; RPKM: Reads per kilobase per million reads; VGT: Vertical gene transfer.
Gene prediction modeling identified 31,987 genes, most of which are supported by cDNA and deep RNA sequencing data.
Referenced (supported by cDNA sequences) or predicted amino acid sequences were aligned using ClustalX program [41].
Although, we cannot formally exclude the possibility that the structure of some genes may have been mispredicted, most human and mouse DANGER gene structures are supported by cDNA sequences.
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