These incubated crystals could then be fished up using a small nylon loop supported on a metal cap, and quickly flash frozen by mounting the cap on the diffractometer equipped with cold stream from liquid nitrogen, ready to shoot with X-rays.
The gels were placed on nylon filters supported by metal grids and cultured for 10 days at the interface with SCC medium and either DMSO vehicle, 2 μM Y27632 or 2 μM BDP5290.
Side handles support the nylon straps comprising Velcro and plastic dowels to hold the neck, limbs, shoulders, and back.
Teflon-racks, placed on the floor of the vessels, supported a 1190 μm nylon mesh (Sefar) as substrate for the plants and two glass slides with a 5 μm grid (N°5, Boraglas) used as a reference to measure root growth.
Putative positive clones were re-spotted on nylon membranes for colony hybridization to confirm true positives.
The S. latifolia BAC library (total of 119,808 colonies) was arrayed on six nylon filters with 18,432 colonies each, and one nylon filter containing 9,216 clones.
"Fawn" focuses on nylon, as in pantyhose.
Investigators looking into the March collapse have focused on nylon slings that broke during the jump.
Briefly, genomic DNA was spotted on nylon membrane, and then baked at 80°C for 2 h.
B. anthracis ∆Sterne spores on nylon were the most difficult to inactivate and to model.
