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While there is a mild induction of the inflammatory response for the 100- and 200-nm topographies, these growth conditions also supported expressions of genes that would be responsible in the prevention of foam cell formation and the removal of foam cells, suggesting potential benefits.
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It was evident that mESCs neither supported expression of EBNA1 as indicated by our initial observations nor EBNA2 as revealed by PCR analysis (Fig. 3C, Table 1).
NSQ50 supported expression of the osteogenic marker Runx2 (Fig. 4).
Multivariate analysis supported expression of HIF-1 α and VEGF-D as significant survival predictors (P = 0.044 and 0.035, respectively).
The objection is embodied by a letter that more than 200 PEN members have signed, "there is a critical difference between staunchly supporting expression that violates the acceptable, and enthusiastically rewarding such expression".
"There is a critical difference between staunchly supporting expression that violates the acceptable, and enthusiastically rewarding such expression," the letter reads.
By supporting expression of two transgenes from a single promoter, this sequence was also expected to allow straightforward detection of the recombination event.
In short: is there not a difference a critical difference between staunchly supporting expression that violates the acceptable and enthusiastically awarding such expression?
All vectors carried the PGK-promoter to support expression in ES-cells.
We computationally tested this hypothesis five ways, and in addition found supporting expression evidence from ESTs (see below).
Taken together, our findings suggest that both eIF4G isoforms can support expression of proteins essential for fitness under a wide variety of growth conditions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com