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In general, sensitivity of sgn3 to growth conditions is such that we observed everything from a near absence of phenotypes to severely dwarfed plants that did not reproduce when grown in different, standard growth chambers that all well supported wild type growth.
However, KHC1-910 does not support wild-type localization of oskar or wild-type streaming.
Minor points: 1) Does the MalF-TM chimera accumulate properly in the forespore, activate σF, and support wild-type sporulation efficiency?
The MalF-TM-SpoIIE chimera does not support wild-type sporulation efficiency and we have added these data to the table in Figure 4 source data 1.
The best model to fit all of the data is that these charge swap mutants support wild-type levels of Cdc13p– Est1p interaction, but the resulting complex is somehow defective in vivo such that it is unable to support wild-type levels of telomerase telomere interaction or telomerase extension.
The fact that FBM neurons lacking scrib and vangl2 failed to migrate in a pk1b morphant host, which has the environmental cues to support wild-type FBM neuron migration, reveals an essential cell-autonomous requirement for these core PCP components in addition to their function in the polarized environment.
Although the efficiency of DNMT3A co-precipitation varied among the clones, the pattern of variation did not correlate with the observed DNA methylation of the Oct3/4 promoter; that is, DNMT3A co-precipitation with the D905R mutant (which has reduced DNA methylation of Oct3/4) is equal to or better than that for several of the other mutants (which support wild-type DNA methylation of Oct3/4).
Substitutions of Glu1.35 by Asp, Gln or Arg were incapable of supporting wild-type OTR agonist binding or signaling.
viciae supports wild-type rates of nitrogen fixation.
Remarkably, a version of the Atf1 protein that is not detectably phosphorylated [40] supports wild-type levels of hotspot recombination under conditions where Atf1 is rate limiting (Figure 3).
Strains containing SpoIIIE-GFP-SsrA* without SspBEc or expressing SspBEc with untagged-SpoIIIE supported wild-type sporulation, chromosome translocation and membrane fission (Supplemental data).
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