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The recovered trees support the split of P. algirus into at least two main clades, which separated around 3.01 ± 0.07 Mya.
We find that for the hemagglutinin gene, a proportion of sequence alignments support the split of subtype B from subtype C after the split between subtypes A-H3N2 and B, in opposition to the topology enforced in our analysis.
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More recent paleogeographical events support the splitting of some leuciscine groups in the Early-Middle Miocene, from 20 to 12 mya, giving rise to most of the current genera.
Molecular phylogenetic studies support the splitting of conifers into two groups: Pinaceae and Coniferales II, corresponding to all conifer families other than Pinaceae (Bowe et al. 2000; Gugerli et al. 2001; Lu et al. 2014).
The studies of Xi et al. [ 115] and Sun et al. [ 116] on the micromorphology of the pollen and leaf epidermis of Neottianthe, respectively, supported the split of N. cucullata s.l.l
Accordingly, results of spectral analyses showed that 1) most of the positions in mitochondrial data supporting the split of Trichobranchidae/Ampharetidae/Alvinellidae are noisy within ingroup and outgroup, and 2) equal in numbers to the splits of Terebellidae/Ampharetidae/Alvinellidae and Pectinaridae/Ampharetidae/Alvinellidae.
A recent analysis of DNA sequences supported the splitting of Sterna into several smaller genera.
In some cases this may produce evidence supporting the splitting of a cluster into more than one species.
The neighbour-net method computes a matrix of distances (much like the neighbour joining method) and produces a network with weights assigned to each split that are proportional to the number of sites that support the split.
The Coralliidae are clearly composed of three lineages, which support the recent split of Corallium into three genera, Corallium, Hemicorallium, and Pleurocorallium (Ardila et al. 2012; Figueroa and Baco 2014).
Since our main purpose in this part of the work was to test if the separation of the mitochondrial lineages occurred in the Pleistocene, any increase in this rate would still support the Pleistocene split of the G. pyrenaicus mitochondrial lineages.
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