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The expression status of 6 of the genes in question was verified via quantitative RT-PCR; the absolute quantifications support the microarray analysis (Table 2).
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JBT supported the microarray analysis.
ZEB1 expression in A549 cells (erlotinib resistant) is two- to three-fold higher than in either of the two EGFR mutant, erlotinib-sensitive cells (H1650 and PC9), supporting the microarray analysis.
SM and JP performed laboratory analyses and data analysis; DRG was responsible for family selection; JBT and JEB supported the microarray analysis; SM wrote the first draft of the manuscript, followed by contributions from remaining authors; SM, JGB and DRT planned and coordinated the research; DRG, JGB and DRT were project leaders.
We thank Barbara Schwager, Mary C. Perianayagam, PhD, and Claudia Rittmüller for their excellent technical assistance, and Ute Wirkner, PhD, for her general support with the microarray analysis.
This notion is supported by the microarray analysis of dystrophic muscles.
This was supported by the microarray analysis which showed that the expressions of some genes involved in iron metabolism were altered.
This is further supported by the microarray analysis data with NB patient tumor samples, showing that high expression of USP7 predicts poor outcomes.
This inference was further supported by the microarray analysis (Fig. 1B) and RT-PCR experiments (Fig. 5), which clearly demonstrate that sven0929 is transcriptionally coregulated with genes involved in CHL biosynthesis.
The result from real-time qPCR supported the results from the microarray analysis (Figure S1).
Importantly, all of the miRNAs for which we were able to amplify PCR products (ten in total) were found to be downregulated in S cells, supporting the accuracy of the microarray analysis (Fig. 2B).
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