Adding a coded indel matrix contributed little additional resolution to the trees, though bootstrap support (BP) was slightly increased for some clades (not shown).
Increasing the content of supported β-nucleating agent slightly increased the Tc, but had no influence on the melting temperatures (Tm) of β-nucleated PP.
This is supported by the observed, slightly increased respiration on UCP2 silencing.
Preliminary analysis (not shown) indicated that including coded gaps slightly increased bootstrap support for some deeper clades, with no major changes in topology.
With a smaller dataset (only one tenrec) the support for the Afrosoricida/Macroscelidea clade slightly increased (PP = 1.00 and BP = 96).
When we removed the single species of coronate, L. unguiculata, the support values for the clade [Cubozoa + Staurozoa] slightly increased (BV = 85), while support values for the clade [Hydrozoa + Discomedusae] decreased (BV = 66).
Ovaries of BicDS103X females appear normal, and while the S103A mutant protein accumulates normally in the oocyte, the BicDS103D protein shows somewhat reduced and the S103F protein slightly increased oocyte accumulation (Supporting Figure S1).
On the other hand, in all datasets including mitochondrial data bootstrap support for the TriTer hypothesis was high, though it slightly decreased with an increasing amount of nuclear data (grey triangles in Figure 3a), and, vice versa, the bootstrap support for the TriAA hypothesis was low, but slightly increased with increasing nuclear data (black triangles in Figure 3a).
The expression of the hemidesmosomal integrins α6 and β4 was slightly increased (see Figure S2 in Supporting Information).
This proportion decreased with grower's age and experienced issues, while slightly increased in presence of multifunctional management and support from family members.
