However, nodal support for this clade was mediocre (0.74), in contrast to the full support recovered by Hahn et al. (2014).
Strong support was recovered for the basal position and monophyly of the subfamily Anophelinae and the tribes Aedini and Sabethini of subfamily Culicinae.
Within the latter, no support is recovered for the generic recognition of Blanfordimys, Lasiopodomys, Neodon, and Phaiomys as suggested by morphology.
This argument is also consistent with the low support values recovered for the majority of the deep nodes using these markers, but it also applies to the cpDNA.
Strong support was recovered for the monilophyte + seed plant relationship by Qiu et al.[ 12] and this relationship is further supported by the large plastid LSC inversion identified in monilophytes and seed plants and not found in lycophytes (Fig. 2 discussed above) [ 5, 39].
A moderate level of support was also recovered for the clustering of lineages E1 and E2.
The CHILERP clade, which received only weak ML bootstrap support, but was recovered for both NRPD2/E2-a (54%) and NRPD2/E2-b (52%), consisted of various V. banksii (section Erpetion) and V. maculata (section Chilenium) lineages, of which one internal mixed species lineage received 100% bootstrap support.
The robust support we recover for Isothrix's sister relationship (nodes 9, 10, and 40 in Figure 3; Table 2) is due to expanded gene sampling in the genus.
We have recovered the same well supported clades that others have recovered for myxogastrids [33], dictyostelids [34], and most well to moderately supported clades of nonfruiting amoebae found consistently in the literature [16], [17], [21], [23], [26], [27], [31], [32], [35], [36].
For many practical applications, immobilization on a solid support allows boronic acid to be easily recovered for repeated use.
