Immunohistochemistry was done using the same specific antibodies as those for Western blotting, in order to support the data obtained by Western blotting as described previously [ 21, 23].
These studies support the data obtained by Hotulainen et al. (2005) showing that cells lacking cofilin exhibit a low G: F actin ratio in reference to control cells.
Although, we have assessed only osteocalcin and DPD as biochemical bone markers of bone remodeling, our results support previous data obtained by bone histomorphometry.
Immunohistochemistry, using the same specific antibodies as employed for Western blotting [ 12, 13], was performed in 8 tumors with distant metastases (M1) and 8 tumors without (M0) to support the data obtained by Western blotting as described previously [ 8].
To support the data obtained by Western blotting, immunohistochemistry was performed with the same antibodies utilized for Western blotting on 3 tumors from patients with lymph node metastases (pN+) and 5 tumors from patients without nodal metastasis (pN-), as described previously [ 20- 22].
MIC of butanolic extract of the fungi ranged from (0.1 20 mg/mL) which supported the data obtained by agar well diffusion assay.
When longer incubation times were used (10 min) ACT, GM1 and the β2 integrin could be detected inside the cell (Fig. 1F), supporting the data obtained by flow cytometry, and demonstrating that ACT, GM1 and the β2 integrin are internalised.
Western blotting with polyclonal rabbit antisera against human trypsin (PRAHT) revealed three bands corresponding to three trypsins in this band supporting the data obtained by MALDI-TOF analysis (Figure 1C).
As shown in figure 5, the relative abundance of several miRNAs in MVs derived from MSCs and HLSCs in respect to their cells of origin, was confirmed by quantitative real time-PCR (qRT-PCR) using the Syber green technique, supporting the data obtained by the MicroRNA Assay Panel.
The importance of circulating factors in modulating health and longevity is supported by data obtained by heterochronic parabiosis experiments in mice [ 5].
