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The read-support filter (8) specifies minimum values for total and strong read support calculated for each candidate in Stage 2. It can help avoid false positives due to misassembly, by requiring that reads directly support the novel sequence at the event breakpoint, and allows the user to adjust the sensitivity to weakly expressed chimeras by filtering predictions based on chimera read coverage.
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Moreover, the inotropic index as a validated marker for the degree of hemodynamic support was calculated for each individual patient [15].
Moreover, the inotropic index as a validated marker for the degree of hemodynamic support was calculated for each individual patient [ 15].
The total of the numbers of clades with >50%, >70%, >80%, >90%, and >95% bootstrap supports was calculated for each of the 20 trees, and the tree showing the largest value was regarded to be the best tree.
A social support index was calculated for each subscale by averaging the survey items in which a higher score indicated a higher perceived availability of social support and a lower score indicated a lower perceived availability of social support in each area.
The overall LSNS social support score was calculated for each patient by summing the 10 questions.
Support values were calculated for each branch split via the Shimodaira-Hasegawa test provided by the –boot option set to 1000 bootstraps for FastTree trees and using the rapid bootstrap for the RaxML tree.
Bremer support values were also calculated for each node in the strict consensus of all MPTs using TreeRot.v2c [69].v2c
The random sampling was performed twenty times for each sampled pool of loci and average bootstrap support for each tree was calculated for each sampling pool.
The broad- and narrow-sense heritability were calculated for each trait (Supporting information Table S3).
Bootstrap support was calculated for the 50%% majority trees.
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