Our client loves us for being their business support backbone mechanism.
H N HSQC, H C HSQC, CBCA CO NH, HNCACB and HBHA CO NH data sets were collected to support backbone assignments, and (H CC CO NH, H CC)(CO NH and HCCH-TOCSY data sets were collected to support sidechain assignments.
The SnS2 nanoflowers anchored on three dimensional porous graphene were easily constructed with nickel foam (NF) as supported backbone through the dip-coating method followed by one-step controllable hydrothermal growth and mild reduction.
Phylogenetic analyses of the 82-taxon data set, using dinoflagellates as an outgroup, resulted in a weakly supported backbone.
CAD contributed substantially to the resolved and highly supported backbone of the phylogenetic tree as evident both from PI plots and from comparing the single gene tree with the total evidence analysis.
We agree with the reviewer that the trees generally have a weakly supported backbone and that the CAD and CCR trees are likely affected by some degree of ancestral paralogy.
The phylogenetic trees presented here, independently of the reconstruction method employed or the partition analyzed (from individual genes to combined data), almost unanimously recovered six principal clades and one independent lineage (Eidolon), variously joined by versions of a poorly supported backbone.
The overall topology of the phylogenetic trees based on the three different data sets (268, 422 and 473 taxa, see Analyses) is very similar and the weakly supported backbone nodes are weak in all trees (Figs. 2, 3, 4 and Additional files 12 and 13).
We chose a minimalist paratope limited to two loops found in a natural camelid heavy-chain antibody (VHH) that binds to ribonuclease A. Ala scanning of the VHH revealed only three "hot spot" side chains and additional four residues important for supporting backbone-mediated interactions.
Support the backbone: In our experience, progress is best achieved when a "backbone organization," keeps the group's work moving forward.
Generally, federal policy has relied, as it should, on private capital and decision-making, but the policy failures of incentive and federal support for backbone infrastructure are many thus our pathetic global ranking.
