To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required.
Average telomere length was measured from genomic DNA using a quantitative real-time PCR (qRT-PCR) assay.
Genome-containing AAV vectors and empty AAV capsid particles were titrated using a quantitative real-time polymerase chain reaction (qPCR 55,56.
We carried out a quantitative Real-Time-PCR using an RT2 Profiler™ plate.
We thank R. Middlebrook and A. Crawley for assistance during experimental set up at Heron Island Research Station and R. Simpson for support with Quantitative Real-Time PCR analysis.
The physico-chemical parameters of the liquid by-products collected over 38 months were supported by quantitative real-time PCR (qPCR) amplifications of functional genes transcripts and a metagenomic approach that describes the archaeal and bacterial community in the MP.
The decrease in liver size was supported by quantitative real-time RT-PCR for the liver specific marker, LFABP (Figure 7G), which revealed that LFABP levels were <10% of normal in mtp2 morphants (0.08+0.036 vs 1.02+0.069, p = 0.025).
These findings are supported by quantitative real-time PCR experiments showing that neuroserpin expression is high, whereas PAI-1 expression is low in both the murine (data not shown) and human brain.
The results were further supported by quantitative real-time RT-PCR analysis, which showed that overexpression of RRM2 significantly increased the expression level of the VEGF mRNA as compared with controls.
Quantitative real time PCR (qPCR) was carried out as described [ 18] using a myIQ single color quantitative Real Time PCR machine (BioRad).
For quantitative real time amplification, SYBR green was used on a Biorad IQ5 system.
