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To achieve carbon-neutral RA production based on photosynthesis without the supply of organic carbon, microalgae could be a good biological material.
These results suggest that insufficient nitrogen sources slow down the growth of Chlorella sp. A similar investigation also showed that an adequate supply of nitrogen sources was essential to promote the growth of the microalgae Chlorella sp. [ 34].
The susceptibility of the microalgae to both polymers was similar.
The biomass of the microalgae was calculated by Eq. (1).
Microalgae cell disruption methods can be mechanical or non-mechanical (thermal, chemical, biological) depending on the characteristics of the microalgae cell wall/membrane and target molecules.
Aquaria received controlled quantities of the microalgae Isochrysis galbana (Prymnesiophyceae) as food.
Moreover, the selected microalgae with Nile red staining exhibited bright yellow fluorescence, which indicated high intracellular lipid content of the microalgae.
Selection would mainly act to increase the fitness of the microalgae in the external environment.
For large-scale production of the microalgae, closed tubular photobioreactors are often used 5.
Centrifugation can harvest more than 90%% of the microalgae, but requires extra energy input [ 9– 12].
Furthermore, the condition factor and protein efficiency ratio of the microalga-fed groups were lower than the CT group.
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