Sentence examples for suppliers recommendations from inspiring English sources

As previously described16, all cell lines were cultured in optimal medium according to the suppliers' recommendations.

The restriction enzyme digestions, ligations and transformations were performed as the suppliers' recommendations.

All cells were maintained and cultured as per suppliers' recommendations.

Cells were cultured according to suppliers' recommendations.

All cell lines were cultured by following the suppliers' recommendations.

Cells were cultured according to suppliers' recommendations, harvested at 90% confluence and prepared for further analyses.

Extraction of total RNA from THP-1 cells was prepared with TRIzol reagent using glycogen as a carrier according to the suppliers' recommendation (Life Technologies).

Samples were centrifuged briefly (1 minute at 13000× g) and analysed by SDS-PAGE on NuPAGE® Novex 4-12% precast polyacrylamide gels (Invitrogen), followed by western blot analysis using ANTI-FLAG M2 monoclonal antibody-horseradish peroxidase conjugate (Sigma) following the suppliers recommendation.

For example, an engineer was asked to "consider" being more open to supplier recommendations and to visit the supplier for a day.

For real time RT-PCR, cDNA synthesis was carried out using the AffinityScript qPCR cDNA Synthesis Kit (Stratagene) according to the supplier recommendations, adding 200 ng total RNA.

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