Sentence examples for suppliers protocol from inspiring English sources

The medium was changed every day, per suppliers' protocol.

The splenic CD11c+ DC were purified using CD11c magnetic beads by suppliers protocol (Miltenyi).

This was achieved using the SMART™ PCR cDNA Synthesis Kit (Clontech, Paris) using the suppliers protocol [43].

NTera2/d1 cells were seeded in a 96-well plate and transfected using IBAfect and MA-enhancer (IBA Biosciences, GmbH) using the suppliers protocol.

1.0 mg streptavidin-coated magnetic beads (M-280 Dynabeads, Dynal Biotech, Oslo, N) were washed twice in B&W buffer (Dynal Biotech, Oslo, N) and biotinylated PCR products were linked to the magnetic beads according to the suppliers protocol.

Emulsion PCR was performed according to the suppliers protocol (LIB-A SV emPCR kit, GS FLX Titanium PicoTiterPlate kit (70×75), GS FLX Titanium XLR 70 Sequencing kit (Roche)).

We performed expression analyses using Human Expression Array U95A version 2 (Affymetrix, Santa Clara, CA) according to the suppliers' protocols.

Each processed sample was assayed in duplicate for pepsinogen C and defensin levels using the suppliers' protocols.

For each liver sample, 10 ng of isolated total RNA were used for real-time quantitative PCR (qPCR) using the QuantiTect® SYBR Green RT-PCR kit (Qiagen GmbH) on a Mastercycler ep realplex apparatus (Eppendorf), following the suppliers' protocols.

The His-tagged proteins and biotinylated PCR-products were detected with anti-His-antibody (Amersham Biosciences, UK), Light Shift Chemiluminescence EMSA Kit (Pierce , USA and SuperSignal West Pico Chemiluminescent Substrate (Pierce , USA, respectively, according to the suppliers' protocols.

Quantification of lactic acid production by melanoma cells was performed after 3-day cultures in 2D or in 3D (30 000 cell density), using as immobilised enzyme biosensor, YSI 2300 STAT Glucose and Lactate analyser (YSI, Yellow Springs, OH, USA), following the suppliers' protocols.