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Follow suppliers' instructions and store where children cannot access.
The Nalm6 (DSMZ, Braunschweig, Germany), Raji (ATCC, Manassas, VA, USA), and K562 (ATCC, Manassas, VA, USA) cell lines were cultured per the suppliers' instructions.
and were grown according to the suppliers instructions.
Primary antibodies used as per suppliers instructions were ERK1/2, phospho-ERK1/2 (Thr202/Tyr204), (Cell Signalling Technology).
NHBE cells were grown and subcultured according to the suppliers instructions in serum-free and hormone supplemented bronchial epithelial growth media (BEGM) (Lonza, Walkersville, Inc).
Luciferase activities and β-galactosidase were individually measured using luciferase assay reagent (Promega, Inc., Madison, WI) and the OpticompI Luminometer (MGM Instruments, Inc. Hamden, CT) according to the suppliers' instructions.
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Cells were grown according to the suppliers' instruction.
See Supplier Instructions for Submitting Invoices.
DNA preparations were quantified using PicoGreen reagents according to the supplier instructions.
according to the supplier instructions.
cDNAs were extracted from the gel slices with agarase (Fermentas) according to the supplier instructions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com