Sentence examples for supplier specifications from inspiring English sources
Rat primary hepatocytes were purchased from Cambrex (Lonza, Walkersville, Maryland) and cultured at 37°C in 5% CO2 according to the supplier specifications.
After treatment with RNAse-free DNAse I (Promega), 1 µg of total RNA was used for cDNA synthesis using Oligo dT 15 (Promega) and the Superscript III Reverse Transcriptase from Invitrogen according to the supplier specifications.
To determine whether Leptospirosis or any other bacterial species may be shed in mouse urine, total genomic DNA was extracted from kidney samples of the same 46 mice, using the Roche High Pure PCR Template Preparation Kit, according to supplier specifications.
Primary antibodies were used as follows: (i) mouse monoclonal 6D6 (Aviva System Biology, USA; dilution 1∶2000) raised against morphine-like compounds (morphine, morphine-3-glucuronide, and M6G, based on supplier specifications and our own experiments) and (ii) rabbit anti-lactoferrin polyclonal antibody (dilution 1∶5000) [33].
Samples were stained using the LIVE/DEAD BacLight kit (Invitrogen), according to supplier specifications and examined with Apotome Fluorescence Microscope (Carl Zeiss International).
All enzymes for cloning, restriction digest and ligation reactions were obtained from Roche Diagnostics (Randburg, South Africa) and used according to supplier specifications.
According to the suppliers' specifications, the lignin was water-soluble, contained 4% sulfur impurities, and had an average Mw of 10.000 Da.
Similarly, Hammond et al. [14] did not present such data but mentioned that all diet preparations were analyzed to confirm that they met the supplier's specifications for certified 5002 rodent diet.
Restriction enzymes were used according to the supplier's specifications (Roche Diagnostics).
α-Amylase was determined using commercial kits from Sigma, (St Louis, Missouri, USA) according to the supplier's specifications.
Its application according to the supplier's specifications (both HF and GC buffer) improved our results from about 50 to 70% positive reactions at first go.
