Sentence examples similar to supplier specification from inspiring English sources

Rat primary hepatocytes were purchased from Cambrex (Lonza, Walkersville, Maryland) and cultured at 37°C in 5% CO2 according to the supplier specifications.

After treatment with RNAse-free DNAse I (Promega), 1 µg of total RNA was used for cDNA synthesis using Oligo dT 15 (Promega) and the Superscript III Reverse Transcriptase from Invitrogen according to the supplier specifications.

To determine whether Leptospirosis or any other bacterial species may be shed in mouse urine, total genomic DNA was extracted from kidney samples of the same 46 mice, using the Roche High Pure PCR Template Preparation Kit, according to supplier specifications.

Primary antibodies were used as follows: (i) mouse monoclonal 6D6 (Aviva System Biology, USA; dilution 1∶2000) raised against morphine-like compounds (morphine, morphine-3-glucuronide, and M6G, based on supplier specifications and our own experiments) and (ii) rabbit anti-lactoferrin polyclonal antibody (dilution 1∶5000) [33].

The values obtained were slightly different in comparison with the suppliers' specifications of 47.0 and 52.6 % respectively, although the total silybin content is similar (97.4 % calculated versus 97.1%% supplier specifications).

Samples were stained using the LIVE/DEAD BacLight kit (Invitrogen), according to supplier specifications and examined with Apotome Fluorescence Microscope (Carl Zeiss International).

All enzymes for cloning, restriction digest and ligation reactions were obtained from Roche Diagnostics (Randburg, South Africa) and used according to supplier specifications.

According to the suppliers' specifications, the lignin was water-soluble, contained 4% sulfur impurities, and had an average Mw of 10.000 Da.

All procedures followed the supplier's specification.

However, for the regenerated CHT samples, the calculated values of DD are 89% and 84% which closely matches the supplier's specification.

When cloning was needed, products of amplification were purified using GFX PCR DNA and Gel Band Purification Kit (Amersham Biosciences, Buckinghamshire, UK), and they were directly cloned into the PMOSBlue vector (Amersham Biosciences) and transformed into MOSBlue competent cells according to the supplier's specification.