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PBZ, heparin, bafilomycin A1 and anti-CD81 antibody were either supplied together with HCVcc to cells during the 4 °C attachment step only, and then removed prior to shifting the temperature to 37 °C; or provided only after the temperature shift.
Polymerase chain reactions (PCR) were carried out in 12.5 µl reactions containing 10 ng of each of the template DNA, 2.5 pmol of each of the primers, 2.5 nmol of each of the dNTPs, 18.6 nmol MgCl2, 0.2 U rTaq DNA polymerase (Takara, Japan) and 1× PCR buffer supplied together with the enzyme.
For this purpose abrasion-resistant cleaning rods are supplied together with the implants.
This quantity of gel was then spread uniformly over the entire surface of each disk using the spreader supplied together with the other components.
Immunohistochemistry was performed using the un-avidin-biotin complex technique named EnVision following the manufacturer's instructions with the reagents supplied together with the kit.
The improvement in axonal regeneration when Schwann cells are supplied together with rod-like physical guidance cues in a nerve conduit is shown in Figure 1.
Similar(48)
Tie alike supplies together with elastic bands.
But the expanding supply, together with equivocal demand, results in ever-lowering prices.
By removing the central hydraulic power supply together with hydraulic pipes, an EHA's reliability and efficiency are greatly improved but its frequency width and stiffness decreased.
The current shortage of tissue and organ donor supply together with the often severe immune complications related to organ transplantation have fueled new scientific disciplines such as tissue engineering and regenerative medicine.
This study has demonstrated that the combined use of alternative water supplies together with water efficient appliances can save up to 77% of total potable water use compared to the average 1990s household water use in the same region considering the location and differing in water use approach.
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