The notes were sent to the interviewees to check and supplement if required.
Yeast was grown in YPD medium or selective MY medium supplemented, if required, with histidine, leucine, methionine and/or uracil to a final concentration of 20 mg/L [ 37].
Supplement with screen shots if required [1-2 pages].
> -wrap-foot> aHofologue of Streptomyces protein (Kaneda et al. 2001) A single colony of the different B. subtilis strains was transferred from a plate to 10 ml Luria-Bertani (LB) medium (if required supplemented with 2 μg/ml erythromycin and 100 μM IPTG) and grown over night (37°C; 300 rpm).
Prior to imaging, cells were starved for 2 h at 37 °C in 0.1% serum supplemented with 50 ng/ml doxycycline if required.
Before starting the trial, efforts will be made, mainly through programme but supplemented with minimal project inputs (if required), to make acceptable quality EmONC services available at the twenty one selected facilities.
If required, media were supplemented with glucose or NaCl at the indicated concentrations.
If required, media were supplemented with antibiotics at the following concentration: erythromycin at 5 µg ml−1 or 10 µg ml−1; tetracycline at 2 µg ml−1.
Probable suicide defined above can be supplemented with possible deaths through suicide if required.
If required, heparin was supplemented to maintain the activated clotting time above 480 seconds and was reversed by protamine at the end of the procedure.
If required, uracil was supplemented to 20 μg/ml while 5′-fluorooritic acid (5′-FOA) was added to 50 μg/ml, in SCVY medium.
