Patients had no signs of renal insufficiency and did not take any vitamin D supplements prior to or in parallel with the investigations.
5,000 cells per cm2 were expanded in parallel with media supplemented with 10% of different serum samples.
DOI: http://dx.doi.org/10.7554/eLife.06963.008 10.7554/eLiFigure63.009 Figure 2 figure supplement 2. The RIDD pathway in parallel with XBP-1 to regulate PVD dendritic arbor development.
Control plates were incubated in parallel with DMEM-low glucose supplemented with 10% heat inactivated FBS and 1% penicillin-streptomycin and subjected to the same fixing and staining procedures.
The flies required for this purpose were generated from backup vials that had excess (∼6 ml) food for larvae and yeast supplement for the adults, and were run in parallel with the experimental vials.
In parallel with the optimization of the reaction procedures, we therefore supplemented the Padlock format with circle probes not requiring target templated ligation.
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It is supplemented with a user-friendly graphical interface easing the tomographic reconstruction work mostly running in parallel with the actual experiment.
For analyzing protein expression, another set of cells was incubated in parallel with L-Methionine and L-cysteine free DMEM medium (GIBCO), supplemented with 200 µCi of [35S]-labeled cysteine-methionine mixture (PerkinElmer), with or without 100 mM sodium chlorate.
When compared to non-DCA-treated SOD1G86R mice, those supplemented with DCA show a decrease in the mRNA expression of Pdk4, Foxo1, and Pparβ/δ in parallel with an induction of Pfk1 and citrate synthase.
After confirmed by PCR and sequencing, the mutants were grown in parallel with wild type Synechocystis in both normal BG11 medium and the BG11 medium supplemented with 0.25% (v/ v) butanol.
