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The results of the present study show that LO can be safely supplemented up to 4% in forage-based diets of dairy cows to enrich milk with potential health beneficial FA (i.e., n-3 FA) without causing any detrimental effects on rumen function, digestion, and milk production.
When daily training or experiments are terminated prematurely, fluids are supplemented up to the normal daily level for that subject.
The mixture was supplemented up to 4 mL with distilled water and was heated at 95°C in a water bath for 60 min.
The remaining 17 to 18 ml blood were supplemented up to 50 ml with lysis buffer containing 0.3 M sucrose, 10 mM Tris-HCl pH 7.5, 5 mM MgCl2 and 1% Triton ×100 (Sigma, Taufkirchen, Germany).
The remaining 16 to 17 ml blood was supplemented up to 50 ml with lysis buffer containing 0.3 M sucrose, 10 mM Tris-HCl pH 7.5, 5 mM MgCl2 and 1% Triton X100 (Sigma, Taufkirchen, Germany).
Then, 6 μl 5RT-Buffer, 1 μl 5 mM dNTP, 0.5 μl RNA Inhibitor, and 1 μl 200 U MML-V RT Enzyme (Promega) were added and supplemented up to a final volume 30 μl with RNase-free water.
Similar(53)
Earlier dose-escalation trials supplementing up to 280 000 IU/w for 28 weeks and up to 40 000 IU for 52 weeks did not report either significant elevations of serum creatinine levels, or calcifications in the kidneys [12], [18].
dH2O was used to supplement up to 20 μl.
dH2O was used to supplement up to 10μL.
Importantly, neither culture media supplemented with up to 27.6 mM Mg2+ ions nor media intentionally adjusted up to alkaline pH 9 induced any detectable adverse effects on HUVEC responses.
The stabilisation fund would be supplemented by up to €250 billion more from the IMF.In addition, the European Central Bank (ECB) said it would purchase government bonds to restore calm to "dysfunctional" markets.
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