We are developing a new tool designed to increase the heme content of our proteins of interest by simply supplementing the expression medium with low concentrations of hemin.
When transgenic mice were fed a fish oil supplemented diet, the expression of adipogenic and glucose uptake genes was increased, leading to reduced plasma glucose concentration.
One millimolar of thiamine was supplemented for the expression of OleTJE.
A number of strategies to harness miRNAs as therapeutics in cancer have been attempted, but they are generally based on the introduction of oligonucleotides to supplement the cellular expression of tumor suppressor miRNAs, or of antisense oligonucleotides to ablate the action of oncogenic miRNAs.
The result shows that the normal mode field of a directional radiator can be obtained by supplementing the normal mode expression of an omnidirectional point source with the directional excitation function, which is dependent on the position and directivity of the radiator.
We further investigated the influence of different methanol concentrations on the expression rate, supplementing the media with casamino acids (CA) to serve as protease- prey" or addition of glycerol, which facilitates the carbon source adaprotease- prey[ 29].
Starting from any existing network supplemented with expression data, the user has to select the attribute field containing the expression information.
To supplement the PCR-based Psg expression studies, we performed 'virtual northern' analysis in silico by screening the public EST database for sequences matching Psg N1 or A-domains and counting the numbers of matches (Fig. 5).
We performed a genome-wide interaction study in rheumatoid arthritis using HLA shared epitope alleles as an anchor in a large population-based study of rheumatoid arthritis in Sweden, and found several new candidate genes, which were replicated by similar evaluation in an independent study from the USA and were supplemented by expression analysis of the genes with highest scores for interaction.
DOI: http://dx.doi.org/10.7554/eLife.02510.018 10.7554/eLiFigure10.019 Figure 4 figure supplement 3. The expression of genes linked to the cell cycle/DNA replication becomes rhythmic in Dicer knockout livers.
DOI: http://dx.doi.org/10.7554/eLife.04205.004 10.7554/eLiFigure05.005 Figure 1 figure supplement 2. The expression patterns of ppk23-GAL4 and ppk29-GAL4 in larval sensory organs and the central nervous system.
